rnascope probe mm-slc17a6-c2 Search Results


superfrost plus slides  (Fisher Scientific)
86
Fisher Scientific superfrost plus slides
Superfrost Plus Slides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probe+mm-slc17a6-c2/plus+slides+superfrost/bio_rxiv__64898__2026__02__08__702109-67-14-17
Average 86 stars, based on 1 article reviews
superfrost plus slides - by Bioz Stars, 2026-10
86/100 stars
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rnascope processing followed acdbio rnascope multiplex fluorescent assay  (Advanced Cell Diagnostics Inc)
99
Advanced Cell Diagnostics Inc rnascope processing followed acdbio rnascope multiplex fluorescent assay
a Timeline showing AAV5-VGluT2-Cre or EV-Cre administration 3 weeks before T9 SCI. Injections indicate the microinjections made to each side of the thoracic spinal cord for site-specific Cre delivery Chronic T9-SCI was followed by intranasal infection of live influenza virus (H3N2 X31). b, c Representative images of <t>RNAscope</t> analysis of TNFR1+ cells in the thoracic SC with EV or VGluT2-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGluT2+ and TNFR1+. Chart represents the proportion of VGluT2+ cells (green) VGluT2+TNFR1+ (dashed green) and all other cells (blue). d Body weight loss of mice for 9 DPI after chronic T9-SCI. (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGluT2-Cre n=12, p values represent significance between AAV5-EV and AAV5-VGluT2, 6 DPI * p= 0.0366, 7 DPI **** p=<0.0001, 8 DPI *** p=0.002, 9 DPI * p=0.0483 using ANOVA, mean ± SEM represented) e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to AAV5-EV-Cre *p=0.0392, AAV5-EV to VGluT2-Cre ** p=0.0039 using ANOVA, mean ± SEM represented) f Spleen weight of mice after 9 DPI weighed at the time of sample collection and processing for flow cytometry analysis. (Uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0052 ANOVA, mean± SEM represented) g Total splenocytes counted at the time of sample collection (Uninjured to PBS * p=0.0407, PBS to XPro1595 * p=0.0496 ANOVA, mean± SEM represented) h Bar graph representing the mean± SEM of CD45+, CD4+, CD8+ and CD44+CD62Llow leukocytes, all cells were gated from single, live, CD45+. i virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGluT2-Cre ns p=0.6392, using student’s t test, mean± SEM represented).
Rnascope Processing Followed Acdbio Rnascope Multiplex Fluorescent Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probe+mm-slc17a6-c2/RNAscope+LS+Multiplex+Fluorescent+Reagent+Kit/bio_rxiv__2024__07__09__602690-190-0-3
Average 99 stars, based on 1 article reviews
rnascope processing followed acdbio rnascope multiplex fluorescent assay - by Bioz Stars, 2026-10
99/100 stars
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gotaq flexi dna polymerase  (Promega)
90
Promega gotaq flexi dna polymerase
a Timeline showing AAV5-VGluT2-Cre or EV-Cre administration 3 weeks before T9 SCI. Injections indicate the microinjections made to each side of the thoracic spinal cord for site-specific Cre delivery Chronic T9-SCI was followed by intranasal infection of live influenza virus (H3N2 X31). b, c Representative images of <t>RNAscope</t> analysis of TNFR1+ cells in the thoracic SC with EV or VGluT2-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGluT2+ and TNFR1+. Chart represents the proportion of VGluT2+ cells (green) VGluT2+TNFR1+ (dashed green) and all other cells (blue). d Body weight loss of mice for 9 DPI after chronic T9-SCI. (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGluT2-Cre n=12, p values represent significance between AAV5-EV and AAV5-VGluT2, 6 DPI * p= 0.0366, 7 DPI **** p=<0.0001, 8 DPI *** p=0.002, 9 DPI * p=0.0483 using ANOVA, mean ± SEM represented) e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to AAV5-EV-Cre *p=0.0392, AAV5-EV to VGluT2-Cre ** p=0.0039 using ANOVA, mean ± SEM represented) f Spleen weight of mice after 9 DPI weighed at the time of sample collection and processing for flow cytometry analysis. (Uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0052 ANOVA, mean± SEM represented) g Total splenocytes counted at the time of sample collection (Uninjured to PBS * p=0.0407, PBS to XPro1595 * p=0.0496 ANOVA, mean± SEM represented) h Bar graph representing the mean± SEM of CD45+, CD4+, CD8+ and CD44+CD62Llow leukocytes, all cells were gated from single, live, CD45+. i virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGluT2-Cre ns p=0.6392, using student’s t test, mean± SEM represented).
Gotaq Flexi Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probe+mm-slc17a6-c2/gotaq+flexi+dna+polymerase/10__7554_slash_elife__43387-207-81-83
Average 90 stars, based on 1 article reviews
gotaq flexi dna polymerase - by Bioz Stars, 2026-10
90/100 stars
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vector genetic reagent dependoparvovirus aav9 cag  (Addgene inc)
94
Addgene inc vector genetic reagent dependoparvovirus aav9 cag
a Timeline showing AAV5-VGluT2-Cre or EV-Cre administration 3 weeks before T9 SCI. Injections indicate the microinjections made to each side of the thoracic spinal cord for site-specific Cre delivery Chronic T9-SCI was followed by intranasal infection of live influenza virus (H3N2 X31). b, c Representative images of <t>RNAscope</t> analysis of TNFR1+ cells in the thoracic SC with EV or VGluT2-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGluT2+ and TNFR1+. Chart represents the proportion of VGluT2+ cells (green) VGluT2+TNFR1+ (dashed green) and all other cells (blue). d Body weight loss of mice for 9 DPI after chronic T9-SCI. (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGluT2-Cre n=12, p values represent significance between AAV5-EV and AAV5-VGluT2, 6 DPI * p= 0.0366, 7 DPI **** p=<0.0001, 8 DPI *** p=0.002, 9 DPI * p=0.0483 using ANOVA, mean ± SEM represented) e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to AAV5-EV-Cre *p=0.0392, AAV5-EV to VGluT2-Cre ** p=0.0039 using ANOVA, mean ± SEM represented) f Spleen weight of mice after 9 DPI weighed at the time of sample collection and processing for flow cytometry analysis. (Uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0052 ANOVA, mean± SEM represented) g Total splenocytes counted at the time of sample collection (Uninjured to PBS * p=0.0407, PBS to XPro1595 * p=0.0496 ANOVA, mean± SEM represented) h Bar graph representing the mean± SEM of CD45+, CD4+, CD8+ and CD44+CD62Llow leukocytes, all cells were gated from single, live, CD45+. i virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGluT2-Cre ns p=0.6392, using student’s t test, mean± SEM represented).
Vector Genetic Reagent Dependoparvovirus Aav9 Cag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probe+mm-slc17a6-c2/pAAV%2ECAG%2EFlex%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100842)/10__7554_slash_elife__75470-211-63-71
Average 94 stars, based on 1 article reviews
vector genetic reagent dependoparvovirus aav9 cag - by Bioz Stars, 2026-10
94/100 stars
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Image Search Results


a Timeline showing AAV5-VGluT2-Cre or EV-Cre administration 3 weeks before T9 SCI. Injections indicate the microinjections made to each side of the thoracic spinal cord for site-specific Cre delivery Chronic T9-SCI was followed by intranasal infection of live influenza virus (H3N2 X31). b, c Representative images of RNAscope analysis of TNFR1+ cells in the thoracic SC with EV or VGluT2-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGluT2+ and TNFR1+. Chart represents the proportion of VGluT2+ cells (green) VGluT2+TNFR1+ (dashed green) and all other cells (blue). d Body weight loss of mice for 9 DPI after chronic T9-SCI. (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGluT2-Cre n=12, p values represent significance between AAV5-EV and AAV5-VGluT2, 6 DPI * p= 0.0366, 7 DPI **** p=<0.0001, 8 DPI *** p=0.002, 9 DPI * p=0.0483 using ANOVA, mean ± SEM represented) e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to AAV5-EV-Cre *p=0.0392, AAV5-EV to VGluT2-Cre ** p=0.0039 using ANOVA, mean ± SEM represented) f Spleen weight of mice after 9 DPI weighed at the time of sample collection and processing for flow cytometry analysis. (Uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0052 ANOVA, mean± SEM represented) g Total splenocytes counted at the time of sample collection (Uninjured to PBS * p=0.0407, PBS to XPro1595 * p=0.0496 ANOVA, mean± SEM represented) h Bar graph representing the mean± SEM of CD45+, CD4+, CD8+ and CD44+CD62Llow leukocytes, all cells were gated from single, live, CD45+. i virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGluT2-Cre ns p=0.6392, using student’s t test, mean± SEM represented).

Journal: bioRxiv

Article Title: Mitigating sTNF/TNFR1 activation on VGluT2+ spinal cord interneurons improves immune function after mid-thoracic spinal cord injury

doi: 10.1101/2024.07.09.602690

Figure Lengend Snippet: a Timeline showing AAV5-VGluT2-Cre or EV-Cre administration 3 weeks before T9 SCI. Injections indicate the microinjections made to each side of the thoracic spinal cord for site-specific Cre delivery Chronic T9-SCI was followed by intranasal infection of live influenza virus (H3N2 X31). b, c Representative images of RNAscope analysis of TNFR1+ cells in the thoracic SC with EV or VGluT2-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGluT2+ and TNFR1+. Chart represents the proportion of VGluT2+ cells (green) VGluT2+TNFR1+ (dashed green) and all other cells (blue). d Body weight loss of mice for 9 DPI after chronic T9-SCI. (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGluT2-Cre n=12, p values represent significance between AAV5-EV and AAV5-VGluT2, 6 DPI * p= 0.0366, 7 DPI **** p=<0.0001, 8 DPI *** p=0.002, 9 DPI * p=0.0483 using ANOVA, mean ± SEM represented) e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to AAV5-EV-Cre *p=0.0392, AAV5-EV to VGluT2-Cre ** p=0.0039 using ANOVA, mean ± SEM represented) f Spleen weight of mice after 9 DPI weighed at the time of sample collection and processing for flow cytometry analysis. (Uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0052 ANOVA, mean± SEM represented) g Total splenocytes counted at the time of sample collection (Uninjured to PBS * p=0.0407, PBS to XPro1595 * p=0.0496 ANOVA, mean± SEM represented) h Bar graph representing the mean± SEM of CD45+, CD4+, CD8+ and CD44+CD62Llow leukocytes, all cells were gated from single, live, CD45+. i virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGluT2-Cre ns p=0.6392, using student’s t test, mean± SEM represented).

Article Snippet: RNAscope processing followed ACDBio RNAScope ® Multiplex Fluorescent Assay manufacturer’s guidelines using probes Mm-TNFRsf1a (cat# 438941-C1) or Mm-Ikbkb (cat# 549481-C1), MmSlc17a6 (cat# 456751-C2), and Mm-SLc32a1 (cat# 319191-C3) from ACDBio.

Techniques: Infection, Virus, RNAscope, Quantitative RT-PCR, Flow Cytometry

a, b Representative images of RNAscope analysis of VGat+ TNFR1+ cells in the thoracic SC with EV or VGat-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGat+ and TNFR1+. Charts are made representing (orange) total VGat+ out of total (blue) and (dashes orange) VGat+TNFR1+. c Body weight loss of mice for 9 DPI after chronic T9-SCI (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGat-Cre n=8, p values represent significance between AAV5-EV and AAV5-VGat, 7 DPI *p= 0.0236, 8 DPI ** p=0.0027, 9 DPI * p=0.0356, using ANOVA, mean ± SEM represented). d Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGat-Cre n=5, uninjured to VGat-Cre *p=0.0280 using ANOVA, mean ± SEM represented). e, f Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (Spleen weight, uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0467 ANOVA, mean± SEM represented. Splenocyte number, uninjured to AAV5-EV-Cre * p=0.0133, AAV5-EV to VGat-Cre *p=0.384 using ANOVA, mean ± SEM represented). g Bar graph representing the differences of CD45+, CD4+, and CD8+ leukocytes All cells were gated from single, live, CD45+. h virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGat-Cre * p=0.0350, using student’s t test, mean± SEM represented).

Journal: bioRxiv

Article Title: Mitigating sTNF/TNFR1 activation on VGluT2+ spinal cord interneurons improves immune function after mid-thoracic spinal cord injury

doi: 10.1101/2024.07.09.602690

Figure Lengend Snippet: a, b Representative images of RNAscope analysis of VGat+ TNFR1+ cells in the thoracic SC with EV or VGat-Cre administration and quantification of successful KD. DAPI cells were counted, followed by VGat+ and TNFR1+. Charts are made representing (orange) total VGat+ out of total (blue) and (dashes orange) VGat+TNFR1+. c Body weight loss of mice for 9 DPI after chronic T9-SCI (Uninjured + X31 n=10 AAV5-EV-Cre n=8, AAV5-VGat-Cre n=8, p values represent significance between AAV5-EV and AAV5-VGat, 7 DPI *p= 0.0236, 8 DPI ** p=0.0027, 9 DPI * p=0.0356, using ANOVA, mean ± SEM represented). d Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGat-Cre n=5, uninjured to VGat-Cre *p=0.0280 using ANOVA, mean ± SEM represented). e, f Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (Spleen weight, uninjured to AAV5-EV-Cre * p=0.0454, AAV5-EV to VGluT2-Cre ** p=0.0467 ANOVA, mean± SEM represented. Splenocyte number, uninjured to AAV5-EV-Cre * p=0.0133, AAV5-EV to VGat-Cre *p=0.384 using ANOVA, mean ± SEM represented). g Bar graph representing the differences of CD45+, CD4+, and CD8+ leukocytes All cells were gated from single, live, CD45+. h virus specific CD8+ T cells at the primary site of infection, the lung (AAV-EV to VGat-Cre * p=0.0350, using student’s t test, mean± SEM represented).

Article Snippet: RNAscope processing followed ACDBio RNAScope ® Multiplex Fluorescent Assay manufacturer’s guidelines using probes Mm-TNFRsf1a (cat# 438941-C1) or Mm-Ikbkb (cat# 549481-C1), MmSlc17a6 (cat# 456751-C2), and Mm-SLc32a1 (cat# 319191-C3) from ACDBio.

Techniques: RNAscope, Quantitative RT-PCR, Flow Cytometry, Virus, Infection

a Cartoon representative of the manipulation of IKKβ knock-down b, c Representative images of RNAscope analysis of VGluT2+ IKKβ cells in the thoracic SC with EV or VGluT2+-Cre administration and quantification of successful KD. Charts are made representing (green) total VGluT2+ out of total (blue) and (dashed green) VGluT2+ IKKβ +. d Body weight loss of mice for 9 DPI after chronic T9-SCI (Uninjured + X31 n=7 AAV5-EV-Cre n=20, AAV5-VGluT2-Cre n=20, p values represent significance between AAV5-EV and AAV5-VGat, 9 DPI * p=0.0288, using ANOVA, mean ± SEM represented). e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to EV-Cre * p=0.0124, AAV5-EV to VGluT2-Cre ** p=0.0089 using ANOVA, mean ± SEM represented). f,g Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (total splenocyte uninjured + X31 to AAV-EV * p=0.0282, uninjured + X31 to VGluT2-Cre ** p=0.0043, using ANOVE, mean ± SEM represented). h Bar graph representing the total amount of CD45+ splenocytes in the spleen (uninjured + X31 to AAV-EV * p=0.0211, uninjured + X31 to VGluT2-Cre ** p=0.0050 using ANOVA, mean ± SEM represented).

Journal: bioRxiv

Article Title: Mitigating sTNF/TNFR1 activation on VGluT2+ spinal cord interneurons improves immune function after mid-thoracic spinal cord injury

doi: 10.1101/2024.07.09.602690

Figure Lengend Snippet: a Cartoon representative of the manipulation of IKKβ knock-down b, c Representative images of RNAscope analysis of VGluT2+ IKKβ cells in the thoracic SC with EV or VGluT2+-Cre administration and quantification of successful KD. Charts are made representing (green) total VGluT2+ out of total (blue) and (dashed green) VGluT2+ IKKβ +. d Body weight loss of mice for 9 DPI after chronic T9-SCI (Uninjured + X31 n=7 AAV5-EV-Cre n=20, AAV5-VGluT2-Cre n=20, p values represent significance between AAV5-EV and AAV5-VGat, 9 DPI * p=0.0288, using ANOVA, mean ± SEM represented). e Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load (Uninjured + X31 n=3, AAV-EV-Cre n=4, AAV5-VGluT2-Cre n=5, uninjured to EV-Cre * p=0.0124, AAV5-EV to VGluT2-Cre ** p=0.0089 using ANOVA, mean ± SEM represented). f,g Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (total splenocyte uninjured + X31 to AAV-EV * p=0.0282, uninjured + X31 to VGluT2-Cre ** p=0.0043, using ANOVE, mean ± SEM represented). h Bar graph representing the total amount of CD45+ splenocytes in the spleen (uninjured + X31 to AAV-EV * p=0.0211, uninjured + X31 to VGluT2-Cre ** p=0.0050 using ANOVA, mean ± SEM represented).

Article Snippet: RNAscope processing followed ACDBio RNAScope ® Multiplex Fluorescent Assay manufacturer’s guidelines using probes Mm-TNFRsf1a (cat# 438941-C1) or Mm-Ikbkb (cat# 549481-C1), MmSlc17a6 (cat# 456751-C2), and Mm-SLc32a1 (cat# 319191-C3) from ACDBio.

Techniques: Knockdown, RNAscope, Quantitative RT-PCR, Flow Cytometry

a,b Representative images of RNAscope analysis of VGat+ IKKβ + cells in the thoracic SC with AAV5-EV-Cre administration (b, top) or AAV5-VGat-Cre administration (b, bottom). Charts are made representing (orange) total VGat+ out of total (blue) and (dashes orange) VGat+ IKKβ +. c Body weight loss of mice for 9 DPI after chronic T9-SCI (* represent uninjured + X31 to AAV5-VGat-Cre, # represent AAV5-EV to VGat-Cre, Uninjured + X31 n=5 AAV5-EV-Cre n=20, AAV5-VGat-Cre n=12, 6 DPI ** p=0.0050, 7 DPI **** p= <0.0001 # p=0.016, 8 DPI *** p=0.0001, ## p=0.0029, 9 DPI *** p=0.0006 # p=0.0128 using ANOVA, mean ± SEM represented) d Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load. e, f Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (splenocytes, uninjured to AAV5-EV-Cre * p=0.0282, AAV5-EV to VGat-Cre *** p=0.0003, using ANOVA, mean ± SEM represented) g Bar graph representing the total amount of CD45+ leukocytes in the spleen (uninjured to AAV5-EV-Cre * p=0.0211, AAV5-EV to VGat-Cre **** p=<0.0001).

Journal: bioRxiv

Article Title: Mitigating sTNF/TNFR1 activation on VGluT2+ spinal cord interneurons improves immune function after mid-thoracic spinal cord injury

doi: 10.1101/2024.07.09.602690

Figure Lengend Snippet: a,b Representative images of RNAscope analysis of VGat+ IKKβ + cells in the thoracic SC with AAV5-EV-Cre administration (b, top) or AAV5-VGat-Cre administration (b, bottom). Charts are made representing (orange) total VGat+ out of total (blue) and (dashes orange) VGat+ IKKβ +. c Body weight loss of mice for 9 DPI after chronic T9-SCI (* represent uninjured + X31 to AAV5-VGat-Cre, # represent AAV5-EV to VGat-Cre, Uninjured + X31 n=5 AAV5-EV-Cre n=20, AAV5-VGat-Cre n=12, 6 DPI ** p=0.0050, 7 DPI **** p= <0.0001 # p=0.016, 8 DPI *** p=0.0001, ## p=0.0029, 9 DPI *** p=0.0006 # p=0.0128 using ANOVA, mean ± SEM represented) d Viral load remaining in the lung at 9 DPI as determined by RT-qPCR for H3N2 viral load. e, f Spleen weight and total live splenocyte count of spleens from mice after 9 DPI. Weighed and analyzed at the time of sample collection and processing for flow cytometry analysis (splenocytes, uninjured to AAV5-EV-Cre * p=0.0282, AAV5-EV to VGat-Cre *** p=0.0003, using ANOVA, mean ± SEM represented) g Bar graph representing the total amount of CD45+ leukocytes in the spleen (uninjured to AAV5-EV-Cre * p=0.0211, AAV5-EV to VGat-Cre **** p=<0.0001).

Article Snippet: RNAscope processing followed ACDBio RNAScope ® Multiplex Fluorescent Assay manufacturer’s guidelines using probes Mm-TNFRsf1a (cat# 438941-C1) or Mm-Ikbkb (cat# 549481-C1), MmSlc17a6 (cat# 456751-C2), and Mm-SLc32a1 (cat# 319191-C3) from ACDBio.

Techniques: RNAscope, Quantitative RT-PCR, Flow Cytometry